Cotranscriptional folding of the lncRNA Xist A-repeats indicates a modular structure

Alisha Jones.

SASDPH5 – SAXS curve of RNA Xist A-repeats (1 repeat)

Xist A-repeat lncRNA 1 repeat
MWexperimental 20 kDa
MWexpected 41 kDa
VPorod 42 nm3
log I(s) 7.29×10-2 7.29×10-3 7.29×10-4 7.29×10-5
Xist A-repeat lncRNA 1 repeat small angle scattering data  s, nm-1
ln I(s)
Xist A-repeat lncRNA 1 repeat Guinier plot ln 7.29×10-2 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
Xist A-repeat lncRNA 1 repeat Kratky plot 1.104 0 3 sRg
Dmax: 16 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of RNA Xist A-repeats (1 repeat) in 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.85 mg/ml was measured at 25°C. One 0.145 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Xist A-repeat lncRNA 1 repeat
Mol. type   RNA
Olig. state   Dimer
Mon. MW   20.3 kDa
Sequence   FASTA