SASDPJ2 – Spiegelmer NOX-A12, 1.0 mg/ml

Spiegelmer NOX-A12

MW^I(0)	17	kDa
MW^expected	15	kDa
V^Porod	21	nm³

log I(s) 3.99×10¹ 3.99×10⁰ 3.99×10^-1 3.99×10^-2

Spiegelmer NOX-A12 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 3.99×10¹ R_g: 2.1 nm 0 (2.1 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 2.2 nm 0 D_max: 7.8 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.7

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.739
0.957

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Spiegelmer NOX-A12 MC-FOLD | MC-SYM model

Synchrotron SAXS data from solutions of Spiegelmer NOX-A12, 1.0 mg/ml in 20 mM HEPES, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.05 mg/ml was measured at 5.8°C. Four successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Tags: X33

Spiegelmer NOX-A12
Mol. type		RNA
Organism		synthetic construct
Olig. state		Monomer
Mon. MW		14.6 kDa
Sequence		FASTA