Structural and Biophysical characterization of N-terminal domain of EccA5 from Mycobacterium tuberculosis.

Jyoti Vishwakarma.

SASDPJ4 – N-terminal domain of EccA5

ESX-5 secretion system protein EccA5
MWI(0) 33 kDa
MWexpected 31 kDa
VPorod 57 nm3
log I(s) 2.46×101 2.46×100 2.46×10-1 2.46×10-2
ESX-5 secretion system protein EccA5 small angle scattering data  s, nm-1
ln I(s)
ESX-5 secretion system protein EccA5 Guinier plot ln 2.46×101 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
ESX-5 secretion system protein EccA5 Kratky plot 1.104 0 √3 sRg
p(r)
ESX-5 secretion system protein EccA5 pair distance distribution function Rg: 2.6 nm 0 Dmax: 7.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
ESX-5 secretion system protein EccA5 DAMMIF model
log I(s)
 s, nm-1
ESX-5 secretion system protein EccA5 GASBOR model
Synchrotron SAXS data from solutions of the N-terminal domain of EccA5 in 20 mM Tris, 150 mM NaCl, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.081 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.30 mg/ml was measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

ESX-5 secretion system protein EccA5 (EccA5-NT)
Mol. type   Protein
Organism   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state   Monomer
Mon. MW   30.9 kDa
 
UniProt   P9WPI1 (1-278)
Sequence   FASTA