Rigid-to-Flexible Transition in a Molecular Brush in a Good Solvent at a Semidilute Concentration

Kang J, Sachse C, Ko C, Schroer M, Vela S, Molodenskiy D, Kohlbrecher J, Bushuev N, Gumerov R, Potemkin I, Jordan R, Papadakis C, Langmuir 38(17):5226-5236 (2022) DOI

SASDPM8 – Molecular brush (PiPOx239-g-PnPrOx14) in a good solvent at concentration c=10 mg/mL

PiPOx239-g-PnPrOx14
MWexperimental 1 kDa
MWexpected 413 kDa
log I(s) 2.36×10-1 2.36×10-2 2.36×10-3 2.36×10-4
PiPOx239-g-PnPrOx14 small angle scattering data  s, nm-1
ln I(s)
PiPOx239-g-PnPrOx14 Guinier plot ln 2.37×10-1 Rg: 8.6 nm 0 (8.6 nm)-2 s2
(sRg)2I(s)/I(0)
PiPOx239-g-PnPrOx14 Kratky plot 1.104 0 √3 sRg
p(r)
PiPOx239-g-PnPrOx14 pair distance distribution function Rg: 9.3 nm 0 Dmax: 34.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
PiPOx239-g-PnPrOx14 DAMMIF model
Synchrotron SAXS data from solutions of Molecular brush (PiPOx239-g-PnPrOx14) in a good solvent at concentration c=10 mg/mL in ethanol, pH 7.3 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 20°C. 20 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

PiPOx239-g-PnPrOx14
Mol. type   Other
Olig. state   Monomer
Mon. MW   413.1 kDa