Argonaute protein SAXS investigation

lirong zheng.

SASDPQ2 – apo-Clostridium butyricum argonaute protein at 10 °C

Clostridium butyricum argonaute protein
MWexperimental 91 kDa
MWexpected 86 kDa
VPorod 188 nm3
log I(s) 1.05×10-1 1.05×10-2 1.05×10-3 1.05×10-4
Clostridium butyricum argonaute protein small angle scattering data  s, nm-1
ln I(s)
Clostridium butyricum argonaute protein Guinier plot ln 1.06×10-1 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Clostridium butyricum argonaute protein Kratky plot 1.104 0 √3 sRg
p(r)
Clostridium butyricum argonaute protein pair distance distribution function Rg: 3.2 nm 0 Dmax: 9.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Clostridium butyricum argonaute protein MOLECULAR DYNAMICS FRAME model
Synchrotron SAXS data from solutions of apo-Clostridium butyricum argonaute protein in 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF, Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.30 mg/ml was measured at 10°C. 20 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Clostridium butyricum argonaute protein
Mol. type   Protein
Olig. state   Monomer
Mon. MW   85.7 kDa
Sequence   FASTA