Methionine gamma lyase fused with S3 domain VGF forms octamers and adheres to tumor cells via binding EGFR

Bondarev N, Bagaeva D, Bazhenov S, Buben M, Bulushova N, Ryzhykau Y, Okhrimenko I, Zagryadskaya Y, Maslov I, Anisimova N, Sokolova D, Kuklin A, Pokrovsky V, Manukhov I, Biochemical and Biophysical Research Communications :149319 (2023) DOI

SASDPR5 – L-methionine gamma-lyase from Clostridium sporogenes

L-methionine gamma-lyase (K272S)
MWexperimental 200 kDa
MWexpected 174 kDa
VPorod 211 nm3
log I(s) 1.48×100 1.48×10-1 1.48×10-2 1.48×10-3
L-methionine gamma-lyase (K272S) small angle scattering data  s, nm-1
ln I(s)
L-methionine gamma-lyase (K272S) Guinier plot ln 1.49×100 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
L-methionine gamma-lyase (K272S) Kratky plot 1.104 0 √3 sRg
p(r)
L-methionine gamma-lyase (K272S) pair distance distribution function Rg: 4.0 nm 0 Dmax: 14.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
L-methionine gamma-lyase (K272S) CHIMERA model
L-methionine gamma-lyase (K272S) CHIMERA model
SANS data from solutions of L-methionine gamma-lyase from Clostridium sporogenes in PBS-D2O (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pD 7.4) performed on the YuMO SANS TOF spectrometer (IBR-2: Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research; Dubna, Russian Federation) with a two He3-fulfilled, 8 independent wire detector system (sample-detector distances of 4.5 m and 12.97 m). The wavelengths used are from 0.05 to 0.8 nm, the contributions of which are separated using time-of-flight technology. One solute concentration of 10.00 mg/ml was measured at 20°C. 9000 successive 0.207 second frames were collected.

The protein of study is L-methionine gamma-lyase from Clostridium sporogenes. The volume fractions of tetramers and octamers in solution are 85% and 15%, respectively.

L-methionine gamma-lyase (K272S) (Cs-MGL)
Mol. type   Protein
Organism   Clostridium sporogenes
Olig. state   Tetramer
Mon. MW   43.5 kDa
 
UniProt   A0A1L3NFS6 (1-400)
Sequence   FASTA
 
PDB ID   5DX5
 
PDB ID   5DX5