SASDPT6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) with 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in phosphate conditions (mixture)
Synchrotron SAXS data from solutions of the N-terminal RNA-binding domainof nucleocapsid protein complexed with 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH 6.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 90.00 μl sample at 4.5 mg/ml was injected at a 0.70 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. 2400 successive 0.995 second frames were collected through the SEC elution. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. 18 sample frames from the SEC peak were selected for averaging using CHROMIXS.
The SAXS data profile displayed in this entry was obtained from the left hand side of the SEC-elution peak that closely resembles SAXS measured from free SL4 RNA (SASBDB entry SASDPM6). The far right hand side of the elution peak generates a profile similar to NTD alone (SASBDB entry SASDPK6). This indicates that in phosphate conditions the complex between SL4 and the NTD protein is not stable in phosphate conditions. Additional SEC-SAXS data (unsubtracted frames) are made available in the full entry zip archive.
s, nm-1
