Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions.

Ohara N, Kawakami N, Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K, J Am Chem Soc (2022) Europe PMC

SASDPV2 – original TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

Fusion protein of LSm and MyoX-coil
MWexperimental 1072 kDa
MWexpected 1066 kDa
log I(s) 3.33×100 3.33×10-1 3.33×10-2 3.33×10-3
Fusion protein of LSm and MyoX-coil small angle scattering data  s, nm-1
ln I(s)
Fusion protein of LSm and MyoX-coil Guinier plot ln 3.34×100 Rg: 9.1 nm 0 (9.1 nm)-2 s2
(sRg)2I(s)/I(0)
Fusion protein of LSm and MyoX-coil Kratky plot 1.104 0 √3 sRg
p(r)
Fusion protein of LSm and MyoX-coil pair distance distribution function Rg: 8.9 nm 0 Dmax: 22.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Fusion protein of LSm and MyoX-coil DAMMIN model
log I(s)
 s, nm-1
Fusion protein of LSm and MyoX-coil PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins) in 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH 8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK, Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 20°C. 30 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Fusion protein of LSm and MyoX-coil (TIP60 protomer)
Mol. type   Protein
Organism   Artificial protein
Olig. state   Other
Mon. MW   17.8 kDa
Sequence   FASTA
 
PDB ID   7EQ9