Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions.

Ohara N, Kawakami N, Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K, J Am Chem Soc (2022) Europe PMC

SASDPW2 – mTIP60-Ba (metal-ion induced 60-mer complex of TIP60 (K67E) mutant with barium ions)

TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions)
Barium ion
MWexperimental 1135 kDa
MWexpected 1074 kDa
log I(s) 3.59×100 3.59×10-1 3.59×10-2 3.59×10-3
TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions) Barium ion small angle scattering data  s, nm-1
ln I(s)
TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions) Barium ion Guinier plot ln 3.59×100 Rg: 9.6 nm 0 (9.6 nm)-2 s2
(sRg)2I(s)/I(0)
TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions) Barium ion Kratky plot 1.104 0 √3 sRg
p(r)
TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions) Barium ion pair distance distribution function Rg: 9.3 nm 0 Dmax: 21.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions) Barium ion DAMMIN model
Synchrotron SAXS data from solutions of mTIP60-Ba (metal-ion induced 60-mer complex of TIP60 (K67E) mutant with barium ions) in 25 mM HEPES, 100 mM NaCl, 5% glycerol, 5 mM BaCl2, pH 8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK, Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 20°C. 30 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions) (mTIP60-Ba)
Mol. type   Protein
Organism   Artificial protein
Olig. state   Other
Mon. MW   17.8 kDa
Sequence   FASTA
 
Barium ion (Ba)
Mol. type   Other
Olig. state   0
Mon. MW   0.1 kDa
Chemical formula