Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions.

Ohara N, Kawakami N, Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K, J Am Chem Soc (2022) Europe PMC

SASDPX2 – TIP60 (K67E) mutant (dimer) with EDTA

TIP60 (K67E) mutant with EDTA
MWexperimental 41 kDa
MWexpected 36 kDa
log I(s) 1.36×10-1 1.36×10-2 1.36×10-3 1.36×10-4
TIP60 (K67E) mutant with EDTA small angle scattering data  s, nm-1
ln I(s)
TIP60 (K67E) mutant with EDTA Guinier plot ln 1.36×10-1 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
TIP60 (K67E) mutant with EDTA Kratky plot 1.104 0 √3 sRg
p(r)
TIP60 (K67E) mutant with EDTA pair distance distribution function Rg: 3.7 nm 0 Dmax: 12 nm

Data validation

Fits and models

log I(s)
 s, nm-1
TIP60 (K67E) mutant with EDTA DAMMIN model
Synchrotron SAXS data from solutions of TIP60 (K67E) in 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH 8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK, Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 20°C. 30 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

TIP60 (K67E) mutant with EDTA (TIP60 (K67E))
Mol. type   Protein
Organism   Artificial protein
Olig. state   Dimer
Mon. MW   17.8 kDa
Sequence   FASTA