| MWexperimental | 21 | kDa |
| MWexpected | 26 | kDa |
| VPorod | 29 | nm3 |
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log I(s)
5.51×101
5.51×100
5.51×10-1
5.51×10-2
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s, nm-1
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Binding by calmodulin is coupled to transient unfolding of the third FF domain of Prp40A.
Díaz Casas A, Cordoba JJ, Ferrer BJ, Balakrishnan S, Wurm JE, Pastrana-Ríos B, Chazin WJ, Protein Sci 32(4):e4606 (2023) Europe PMCSASDPX6 – Calmodulin bound to the FF3 domain of pre-mRNA-processing factor 40 homolog A (Prp40A)
Calmodulin-1Pre-mRNA-processing factor 40 homolog A
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Fits and models
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Synchrotron SAXS data from solutions of calmodulin bound to the FF3 domain of pre-mRNA-processing factor 40 homolog A in 50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH 7.4 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS: Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 30.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex LW-803 column at 25°C. 800 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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