Structural analysis of the Candida albicans mitochondrial DNA maintenance factor Gcf1p reveals a dynamic DNA-bridging mechanism.

Tarrés-Solé A, Battistini F, Gerhold JM, Piétrement O, Martínez-García B, Ruiz-López E, Lyonnais S, Bernadó P, Roca J, Orozco M, Le Cam E, Sedman J, Solà M, Nucleic Acids Res (2023) Europe PMC

SASDPY5 – Gcf1p protein

Gcf1p
MWexperimental 28 kDa
MWexpected 26 kDa
VPorod 80 nm3
log I(s) 2.34×101 2.34×100 2.34×10-1 2.34×10-2
Gcf1p small angle scattering data  s, nm-1
ln I(s)
Gcf1p Guinier plot ln 2.35×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Gcf1p Kratky plot 1.104 0 √3 sRg
Dmax: 14.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Gcf1p EOM/RANCH model
Gcf1p EOM/RANCH model
Gcf1p EOM/RANCH model
Gcf1p EOM/RANCH model
Synchrotron SAXS data from solutions of Gcf1p protein in 50 mM Tris, 750 mM NaCl, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.3 and 10 mg/ml were measured at 10°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Sample detector distance = UNKNOWN

Gcf1p
Mol. type   Protein
Organism   Candida albicans (strain SC5314 / ATCC MYA-2876)
Olig. state   Monomer
Mon. MW   26.0 kDa
 
UniProt   Q59QB8 (25-245)
Sequence   FASTA