BTB domains: A structural view of evolution, multimerization, and protein-protein interactions.

Bonchuk A, Balagurov K, Georgiev P, Bioessays :e2200179 (2022) Europe PMC

SASDPZ6 – BTB domain of longitudinals lacking protein (LOLA) at 3.0 mg/ml

Longitudinals lacking protein, isoform G
MWI(0) 103 kDa
MWexpected 92 kDa
VPorod 263 nm3
log I(s) 7.58×10-2 7.58×10-3 7.58×10-4 7.58×10-5
Longitudinals lacking protein, isoform G small angle scattering data  s, nm-1
ln I(s)
Longitudinals lacking protein, isoform G Guinier plot ln 7.58×10-2 Rg: 4.8 nm 0 (4.8 nm)-2 s2
(sRg)2I(s)/I(0)
Longitudinals lacking protein, isoform G Kratky plot 1.104 0 3 sRg
Dmax: 24 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of the BTB domain of LOLA in 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM DTT, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 10°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Possibly aggregated.

Longitudinals lacking protein, isoform G (LOLA)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Hexamer
Mon. MW   15.3 kDa
 
UniProt   P42283 (1-120)
Sequence   FASTA