| MWexperimental | 433 | kDa |
| MWexpected | 224 | kDa |
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log I(s)
8.50×103
8.50×102
8.50×101
8.50×100
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s, nm-1
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An Anomalous Small-Angle X-Ray Scattering Study of the Formation of Iron Clusters in the Inner Cavity of the Ferritin-Like Protein Dps
Gordienko A, Mozhaev A, Gibizova V, Dadinova L, Moscow University Physics Bulletin 77(6):858-867 (2023) DOISASDPZ7 – DNA protection during starvation protein (Dps) loaded with FeSO4⋅7H2O in the amount corresponding to 2000 iron atoms per dodecamer. Anomalous SAXS spanning the X-ray Fe-absorption edge 7.100-7.133 keV.
DNA protection during starvation protein|
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Fits and models
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Synchrotron SAXS data from solutions of DNA protection during starvation protein (Dps) loaded with FeSO4⋅7H2O in the amount corresponding to 2000 iron atoms per dodecamer in 50 mM NaCl, 0.5 mM EDTA, 50 mM Tris-HCl, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.173818 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 10°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The SAXS data in this entry show Dps loaded with FeSO4⋅7H2O in the amount corresponding to 2000 iron atoms per dodecamer measured at 7.133 keV and the corresponding model fits, in addition to the fits to the resonant and non-resonant anomalous SAXS data curves extracted across the Fe X-ray absorption edge (7.100-7.133 keV). The full anomalous SAXS data series, models and fits are made available in the full entry zip archive |
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