Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2.

Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M, Nat Commun 15(1):5241 (2024) Europe PMC

SASDQ26 – Retinoic acid-induced protein 2 (RAI2: 303-362) M1 mutant L319A, S320A

Retinoic acid-induced protein 2 (303-362: L319A, S320A)
MWI(0) 7 kDa
MWexpected 7 kDa
VPorod 10 nm3
log I(s) 4.50×10-3 4.50×10-4 4.50×10-5 4.50×10-6
Retinoic acid-induced protein 2 (303-362: L319A, S320A) small angle scattering data  s, nm-1
ln I(s)
Retinoic acid-induced protein 2 (303-362: L319A, S320A) Guinier plot ln 4.51×10-3 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Retinoic acid-induced protein 2 (303-362: L319A, S320A) Kratky plot 1.104 0 √3 sRg
p(r)
Retinoic acid-induced protein 2 (303-362: L319A, S320A) pair distance distribution function Rg: 2.5 nm 0 Dmax: 9.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Retinoic acid-induced protein 2 (RAI2: 303-362) M1 mutant L319A, S320A in 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.79 mg/ml was measured at 20°C. 40 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Retinoic acid-induced protein 2 (303-362: L319A, S320A) (RAI2 (303-362) M1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   6.9 kDa
 
UniProt   Q9Y5P3 (303-362)
Sequence   FASTA