MWexperimental | 55 | kDa |
MWexpected | 87 | kDa |
VPorod | 102 | nm3 |
log I(s)
2.25×101
2.25×100
2.25×10-1
2.25×10-2
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s, nm-1
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Synchrotron SAXS data from solutions of minimal proline dehydrogenase domain of proline utilization A (SmPutADeltaAlpha2) in 25 mM HEPES pH 7.6, 150 mM NaCl, and 1mM TCEP, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1234 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.10 mg/ml was measured at 20°C. 30 successive 0.330 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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