Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2.

Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M, Nat Commun 15(1):5241 (2024) Europe PMC

SASDQ76 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362)

C-terminal-binding protein 1
Retinoic acid-induced protein 2 (303-362)
MWI(0) 130 kDa
MWexpected 198 kDa
VPorod 889 nm3
log I(s) 8.47×10-2 8.47×10-3 8.47×10-4 8.47×10-5
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362) small angle scattering data  s, nm-1
ln I(s)
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362) Guinier plot ln 8.47×10-2 Rg: 7.4 nm 0 (7.4 nm)-2 s2
(sRg)2I(s)/I(0)
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362) Kratky plot 1.104 0 3 sRg
p(r)
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362) pair distance distribution function Rg: 7.5 nm 0 Dmax: 25 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) in 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.79 mg/ml was measured at 20°C. 40 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

C-terminal-binding protein 1 (CtBP1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   47.7 kDa
 
UniProt   Q13363 (1-440)
Sequence   FASTA
 
Retinoic acid-induced protein 2 (303-362) (RAI2 (303-362))
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   7.0 kDa
 
UniProt   Q9Y5P3 (303-362)
Sequence   FASTA