Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2.

Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M, Nat Commun 15(1):5241 (2024) Europe PMC

SASDQA6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M1+M2 mutant L319A, S320A, L345A, S346A

C-terminal-binding protein 1
Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A)
MWI(0) 31 kDa
MWexpected 198 kDa
VPorod 352 nm3
log I(s) 2.33×10-2 2.33×10-3 2.33×10-4 2.33×10-5
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) small angle scattering data  s, nm-1
ln I(s)
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) Guinier plot ln 2.33×10-2 Rg: 4.8 nm 0 (4.8 nm)-2 s2
(sRg)2I(s)/I(0)
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) Kratky plot 1.104 0 3 sRg
p(r)
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) pair distance distribution function Rg: 4.9 nm 0 Dmax: 15.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M1+M2 mutant L319A, S320A, L345A, S346A in 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.60 mg/ml was measured at 20°C. 40 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

C-terminal-binding protein 1 (CtBP1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   47.7 kDa
 
UniProt   Q13363 (1-440)
Sequence   FASTA
 
Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) (RAI2 (303-362) M1+M2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   6.8 kDa
 
UniProt   Q9Y5P3 (303-362)
Sequence   FASTA