Structural insights of the elongation factor EF-Tu complexes in protein translation of Mycobacterium tuberculosis.

Zhan B, Gao Y, Gao W, Li Y, Li Z, Qi Q, Lan X, Shen H, Gan J, Zhao G, Li J, Commun Biol 5(1):1052 (2022) Europe PMC

SASDQB5 – Elongation factor Tu from Mycobacterium tuberculosis

Elongation factor Tu
MWexperimental 40 kDa
MWexpected 44 kDa
VPorod 94 nm3
log I(s) 5.63×10-3 5.63×10-4 5.63×10-5 5.63×10-6
Elongation factor Tu small angle scattering data  s, nm-1
ln I(s)
Elongation factor Tu Guinier plot ln 5.63×10-3 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
Elongation factor Tu Kratky plot 1.104 0 3 sRg
p(r)
Elongation factor Tu pair distance distribution function Rg: 2.9 nm 0 Dmax: 9.0 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Elongation factor Tu DAMMIN model

Synchrotron SAXS data from solutions of elongation factor Tu in 20 mM Tris, 100 mM NaCl, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.13 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The bead model shown in this entry is the volume and bead occupancy corrected model based on the alignment of an individual model cohort. It does not represent the corresponding fit to the data as displayed in this entry. CAUTION: Possibly aggregated. CAUTION: Possibly background over-subtracted.

Elongation factor Tu
Mol. type   Protein
Organism   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state   Monomer
Mon. MW   43.6 kDa
 
UniProt   P9WNN1 (1-396)
Sequence   FASTA