Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2.

Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M, Nat Commun 15(1):5241 (2024) Europe PMC

SASDQB6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) deletion mutant ΔE331-A341

C-terminal-binding protein 1
Retinoic acid-induced protein 2 (303-362: ΔE331-A341)
MWI(0) 64 kDa
MWexpected 197 kDa
VPorod 912 nm3
log I(s) 4.75×10-2 4.75×10-3 4.75×10-4 4.75×10-5
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: ΔE331-A341) small angle scattering data  s, nm-1
ln I(s)
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: ΔE331-A341) Guinier plot ln 4.75×10-2 Rg: 7.2 nm 0 (7.2 nm)-2 s2
(sRg)2I(s)/I(0)
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: ΔE331-A341) Kratky plot 1.104 0 3 sRg
p(r)
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: ΔE331-A341) pair distance distribution function Rg: 7.6 nm 0 Dmax: 28 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) deletion mutant ΔE331-A341 in 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.60 mg/ml was measured at 20°C. 40 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

C-terminal-binding protein 1 (CtBP1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   47.7 kDa
 
UniProt   Q13363 (1-440)
Sequence   FASTA
 
Retinoic acid-induced protein 2 (303-362: ΔE331-A341) (RAI2 (303-362) d11)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   5.8 kDa
 
UniProt   Q9Y5P3 (303-362)
Sequence   FASTA