Structural insights of the elongation factor EF-Tu complexes in protein translation of Mycobacterium tuberculosis.

Zhan B, Gao Y, Gao W, Li Y, Li Z, Qi Q, Lan X, Shen H, Gan J, Zhao G, Li J, Commun Biol 5(1):1052 (2022) Europe PMC

SASDQC5 – Elongation factor Ts from Mycobacterium tuberculosis

Elongation factor Ts
MWexperimental 28 kDa
MWexpected 29 kDa
VPorod 46 nm3
log I(s) 2.58×10-2 2.58×10-3 2.58×10-4 2.58×10-5
Elongation factor Ts small angle scattering data  s, nm-1
ln I(s)
Elongation factor Ts Guinier plot ln 2.59×10-2 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Elongation factor Ts Kratky plot 1.104 0 √3 sRg
p(r)
Elongation factor Ts pair distance distribution function Rg: 2.8 nm 0 Dmax: 10.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Elongation factor Ts DAMMIN model
Synchrotron SAXS data from solutions of elongation factor Ts in 20 mM Tris, 100 mM NaCl, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.13 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The bead model shown in this entry is the volume and bead occupancy corrected model based on the alignment of an individual model cohort. It does not represent the corresponding fit to the data as displayed in this entry.

Elongation factor Ts
Mol. type   Protein
Organism   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state   Monomer
Mon. MW   28.8 kDa
 
UniProt   P9WNM1 (1-271)
Sequence   FASTA