| MWexperimental | 87 | kDa |
| MWexpected | 68 | kDa |
| VPorod | 206 | nm3 |
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log I(s)
3.69×10-2
3.69×10-3
3.69×10-4
3.69×10-5
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s, nm-1
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Visualizing RNA conformational and architectural heterogeneity in solution.
Ding J, Lee YT, Bhandari Y, Schwieters CD, Fan L, Yu P, Tarosov SG, Stagno JR, Ma B, Nussinov R, Rein A, Zhang J, Wang YX, Nat Commun 14(1):714 (2023) Europe PMCSASDQG7 – Thermoanearobacter tengcongensis (Tte) fecB RNA riboswitch aptamer domain (rCbl)
Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain|
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Fits and models
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Synchrotron SAXS data from solutions of the fecB RNA riboswitch aptamer domain (rCbl) in 50 mM MES pH 6.0, 10 mM KCl, 1 mM MgCl2, were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS; Argonne National Laboratory; Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 1.9 m and at a wavelength of λ = 0.0932 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.07 mg/ml was measured at 10°C. 225 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The molecular weight was estimated based on the volume of correlation (Vc) method for RNA. The molecular weight for the monomer RNA is 68.5kDa. The molecular weight result from SAXS confirms that the sample consists of monomer and multimeric species. |
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