Synchrotron SAXS data from solutions of G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N104 in 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample at 9.5 mg/ml was injected at a 0.30 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 20°C. 540 successive 0.995 second frames were collected through the entire SEC elution profile, where 26 frames were processed through the sample elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The molecule contains residues E105-D382 of G. gallus TRPV4, which includes a PIP2 binding site (K107-R111), a proline-rich region (T121-V134) and an Ankryin repeat domain. The individual, unsubtracted SEC-SAXS data frames are made available in the full entry zip archive.
s, nm-1
