Borna Disease Virus 1 Phosphoprotein Forms a Tetramer and Interacts with Host Factors Involved in DNA Double-Strand Break Repair and mRNA Processing.

Tarbouriech N, Chenavier F, Kawasaki J, Bachiri K, Bourhis JM, Legrand P, Freslon LL, Laurent EMN, Suberbielle E, Ruigrok RWH, Tomonaga K, Gonzalez-Dunia D, Horie M, Coyaud E, Crépin T, Viruses 14(11) (2022) Europe PMC

SASDQP5 – Phosphoprotein of Borna disease virus

Phosphoprotein
MWexperimental 90 kDa
MWexpected 90 kDa
VPorod 225 nm3
log I(s) 1.69×10-2 1.69×10-3 1.69×10-4 1.69×10-5
Phosphoprotein small angle scattering data  s, nm-1
ln I(s)
Phosphoprotein Guinier plot ln 1.69×10-2 Rg: 6.1 nm 0 (6.1 nm)-2 s2
(sRg)2I(s)/I(0)
Phosphoprotein Kratky plot 1.104 0 √3 sRg
p(r)
Phosphoprotein pair distance distribution function Rg: 6.4 nm 0 Dmax: 21.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Phosphoprotein of Borna disease virus Rg histogram Rg, nm
Phosphoprotein CUSTOM IN-HOUSE model
Phosphoprotein CUSTOM IN-HOUSE model
Phosphoprotein CUSTOM IN-HOUSE model
Phosphoprotein CUSTOM IN-HOUSE model
Synchrotron SAXS data from solutions of phosphoprotein of Borna disease virus in 20 mM HEPES, 150 mM NaCl, 5 mM β- mercaptoethanol, pH 7.5 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 4.6 mg/ml was injected at a 0.30 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 20°C. 540 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Phosphoprotein
Mol. type   Protein
Organism   Borna disease virus (strain V)
Olig. state   Tetramer
Mon. MW   22.5 kDa
 
UniProt   P0C799 (1-201)
Sequence   FASTA