MWexperimental | 45 | kDa |
MWexpected | 46 | kDa |
VPorod | 78 | nm3 |
log I(s)
4.67×10-2
4.67×10-3
4.67×10-4
4.67×10-5
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s, nm-1
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Rg, nm
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Synchrotron SAXS data from solutions of His-Tagged DNA repair protein RAD51 homolog 1 [F86E, A89E] in complex with the His-tagged fourth BRC repeat of BRC4 in 20 mM HEPES, 200 mM Na2SO4, 5% glycerol, 0.1 mM EDTA, pH 8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.095373 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 6.1 mg/ml was injected at a 0.07 ml/min flow rate onto a GE Superdex 200 Increase 3.2/300 column at 15°C. 36 successive frames were collected through the sample elution peak (from a total of 599 frames). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Experimental molecular weight has been obtained through static light scattering (SLS). |
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