Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2.

Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M, Nat Commun 15(1):5241 (2024) Europe PMC

SASDQW5 – C-terminal-binding protein 1 (CtBP1)

C-terminal-binding protein 1
MWI(0) 161 kDa
MWexpected 191 kDa
VPorod 279 nm3
log I(s) 1.04×10-1 1.04×10-2 1.04×10-3 1.04×10-4
C-terminal-binding protein 1 small angle scattering data  s, nm-1
ln I(s)
C-terminal-binding protein 1 Guinier plot ln 1.05×10-1 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
C-terminal-binding protein 1 Kratky plot 1.104 0 √3 sRg
p(r)
C-terminal-binding protein 1 pair distance distribution function Rg: 4.3 nm 0 Dmax: 14 nm

Data validation

Fits and models

log I(s)
 s, nm-1
C-terminal-binding protein 1 CORAL model
Synchrotron SAXS data from solutions of C-terminal-binding protein 1 (CtBP1) in 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.40 mg/ml was measured at 20°C. 40 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

C-terminal-binding protein 1 (CtBP1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   47.7 kDa
 
UniProt   Q13363 (1-440)
Sequence   FASTA
 
PDB ID   6CDF