Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS).

Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D, Commun Biol 6(1):1057 (2023) Europe PMC

SASDQX8 – Bovine serum albumin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Albumin
MWexperimental 66 kDa
MWexpected 66 kDa
log I(s) 1.18×10-2 1.18×10-3 1.18×10-4 1.18×10-5
Albumin small angle scattering data  s, nm-1
ln I(s)
Albumin Guinier plot ln 1.18×10-2 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Albumin Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Scattering data from solutions of bovine serum albumin in phosphate buffered saline, pH 7.4 were collected using a AGIPD-1Mpix detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.13 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

BSA solutions were measured on the European XFEL using a "reservoir" to inject the sample into an in-vacuum liquid jet delivery system. The intensity at low angle increases due to imprecise background subtraction (background varying between sample and buffer measurements and/or the presence of aggregates.)

Albumin (BSA)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   66.4 kDa
 
UniProt   P02769 (25-607)
Sequence   FASTA