Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS).

Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D, Commun Biol 6(1):1057 (2023) Europe PMC

SASDQZ8 – Apoferritin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Ferritin light chain
MWexperimental 480 kDa
MWexpected 479 kDa
log I(s) 3.04×102 3.04×101 3.04×100 3.04×10-1
Ferritin light chain small angle scattering data  s, nm-1
ln I(s)
Ferritin light chain Guinier plot ln 3.05×102 Rg: 5.8 nm 0 (5.8 nm)-2 s2
(sRg)2I(s)/I(0)
Ferritin light chain Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Scattering data from solutions of Apoferritin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL in phosphate buffered saline, pH 7.4 were collected using a AGIPD-1Mpix detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.13 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Apoferritin solutions were measured on the European XFEL using a "reservoir" to inject the sample into an in-vacuum liquid jet delivery system.

Ferritin light chain
Mol. type   Protein
Organism   Equus caballus
Olig. state   24-mer
Mon. MW   20.0 kDa
 
UniProt   P02791 (1-175)
Sequence   FASTA