Invariant BECN1 CXXC motifs bind Zn(2+) and regulate structure and function of the BECN1 intrinsically disordered region.

Mukhopadhyay S, Subedi S, Hopkins JB, Ugrinov A, Chakravarthy S, Colbert CL, Sinha SC, Autophagy :1-17 (2023) Europe PMC

SASDR22 – Beclin-1 amino acids 1-150 with two cysteine motifs, along with a C-terminal tyrosine analysed using EFA in BIOXTAS RAW software, component 1

Beclin-1
MWI(0) 21 kDa
MWexpected 17 kDa
VPorod 49 nm3
log I(s) 1.61×100 1.61×10-1 1.61×10-2 1.61×10-3
Beclin-1 small angle scattering data  s, nm-1
ln I(s)
Beclin-1 Guinier plot ln 1.62×100 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Beclin-1 Kratky plot 1.104 0 √3 sRg
p(r)
Beclin-1 pair distance distribution function Rg: 2.9 nm 0 Dmax: 12 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Beclin-1 amino acids 1-150 with two cysteine motifs, along with a C-terminal tyrosine analysed using EFA in BIOXTAS RAW software, component 1 Rg histogram Rg, nm
Beclin-1 EOM/RANCH model
Beclin-1 EOM/RANCH model
Beclin-1 EOM/RANCH model
Beclin-1 EOM/RANCH model
Beclin-1 EOM/RANCH model
Beclin-1 EOM/RANCH model
Beclin-1 EOM/RANCH model
Synchrotron SAXS data from solutions of Beclin-1 amino acids 1-150 with two cysteine motifs, analysed using evolving factor analysis (EFA component 1), in 50 mM Tris, 300 mM NaCl, pH 8 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 22°C. 394 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The experimental molecular weight was determined using SEC-MALS.

Beclin-1 (BECN1(1-150)Y)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   17.2 kDa
 
UniProt   Q14457 (1-150)
Sequence   FASTA