Vibrio cholerae's ToxRS bile sensing system.

Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T, Elife 12 (2023) Europe PMC

SASDR25 – Periplasmic domain of cholera toxin transcriptional activator ToxR

Cholera toxin transcriptional activator
MWexperimental 11 kDa
MWexpected 12 kDa
VPorod 16 nm3
log I(s) 1.52×102 1.52×101 1.52×100 1.52×10-1
Cholera toxin transcriptional activator small angle scattering data  s, nm-1
ln I(s)
Cholera toxin transcriptional activator Guinier plot ln 1.53×102 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
Cholera toxin transcriptional activator Kratky plot 1.104 0 √3 sRg
p(r)
Cholera toxin transcriptional activator pair distance distribution function Rg: 1.7 nm 0 Dmax: 6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Cholera toxin transcriptional activator PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of ToxRp in 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 10 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. 4800 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cholera toxin transcriptional activator (ToxRp)
Mol. type   Protein
Organism   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Olig. state   Monomer
Mon. MW   11.6 kDa
 
UniProt   P15795 (201-294)
Sequence   FASTA
 
PDB ID   7NN6