Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis.

Wang H, Xie C, Deng B, Ding J, Li N, Kou Z, Jin M, He J, Wang Q, Wen H, Zhang J, Zhou Q, Chen S, Chen X, Yuan TF, Zhu S, Nat Struct Mol Biol (2024) Europe PMC

SASDR32 – Human derived autoantibody mAb2G7 at pH 8.0

Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL
MWexperimental 123 kDa
MWexpected 154 kDa
VPorod 260 nm3
log I(s) 4.10×101 4.10×100 4.10×10-1 4.10×10-2
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH Human derived autoantibody mAb2G7 light chain, mAb2G7 VL small angle scattering data  s, nm-1
ln I(s)
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH Human derived autoantibody mAb2G7 light chain, mAb2G7 VL Guinier plot ln 4.11×101 Rg: 5.0 nm 0 (5.0 nm)-2 s2
(sRg)2I(s)/I(0)
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH Human derived autoantibody mAb2G7 light chain, mAb2G7 VL Kratky plot 1.104 0 √3 sRg
p(r)
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH Human derived autoantibody mAb2G7 light chain, mAb2G7 VL pair distance distribution function Rg: 5.1 nm 0 Dmax: 16.0 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of human derived autoantibody mAb2G7 in phosphate buffered saline, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.60 mg/ml was measured at 10°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH
Mol. type   Protein
Olig. state   Dimer
Mon. MW   51.3 kDa
Sequence   FASTA
 
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL
Mol. type   Protein
Olig. state   Dimer
Mon. MW   25.7 kDa
Sequence   FASTA