Vibrio cholerae's ToxRS bile sensing system.

Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T, Elife 12 (2023) Europe PMC

SASDR35 – Periplasmic domain of cholera transmembrane regulatory protein ToxS

Transmembrane regulatory protein ToxS
MWexperimental 37 kDa
MWexpected 37 kDa
VPorod 55 nm3
log I(s) 9.16×101 9.16×100 9.16×10-1 9.16×10-2
Transmembrane regulatory protein ToxS small angle scattering data  s, nm-1
ln I(s)
Transmembrane regulatory protein ToxS Guinier plot ln 9.17×101 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Transmembrane regulatory protein ToxS Kratky plot 1.104 0 √3 sRg
p(r)
Transmembrane regulatory protein ToxS pair distance distribution function Rg: 2.3 nm 0 Dmax: 7.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Transmembrane regulatory protein ToxS ALPHAFOLD model
Synchrotron SAXS data from solutions of ToxSp in 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 10 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. 4800 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Transmembrane regulatory protein ToxS (ToxSp)
Mol. type   Protein
Organism   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Olig. state   Dimer
Mon. MW   18.7 kDa
 
UniProt   P24003 (25-173)
Sequence   FASTA