| MWexperimental | 30 | kDa |
| MWexpected | 49 | kDa |
| VPorod | 51 | nm3 |
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log I(s)
3.07×102
3.07×101
3.07×100
3.07×10-1
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s, nm-1
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Vibrio cholerae's ToxRS bile sensing system.
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T, Elife 12 (2023) Europe PMCSASDR45 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS
Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxS
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Fits and models
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Synchrotron SAXS data from solutions of the ToxRp/ToxSp complex in 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 10 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. 4800 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The dimer of ToxSp dissociates on the formation of the complex such that one ToxSp molecule binds to ToxRp. |
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