Wang H,
Xie C,
Deng B,
Ding J,
Li N,
Kou Z,
Jin M,
He J,
Wang Q,
Wen H,
Zhang J,
Zhou Q,
Chen S,
Chen X,
Yuan TF,
Zhu S,
Nat Struct Mol Biol
(2024)
Europe PMC
SASDR62 – Human GluN1-GluN2A NMDA receptor in complex with human derived autoantibody mAb2G7 at pH 8.0
Synchrotron SAXS data from solutions of human GluN1-GluN2A NMDA receptor in complex with human derived autoantibody mAb2G7 in 150 mM NaCl, 0.1% digitonin, 5 µM cholesteryl hemisuccinate (TRIS salt), 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.60 mg/ml was measured at 10°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.