An Arg/Ala-rich helix in the N-terminal region of M. tuberculosis FtsQ is a potential membrane anchor of the Z-ring.

Smrt ST, Escobar CA, Dey S, Cross TA, Zhou HX, Commun Biol 6(1):311 (2023) Europe PMC

SASDRA6 – Cell division protein FtsQ N-terminal soluble domain

Cell division protein FtsQ
MWexperimental 16 kDa
MWexpected 11 kDa
VPorod 21 nm3
log I(s) 2.22×10-2 2.22×10-3 2.22×10-4 2.22×10-5
Cell division protein FtsQ small angle scattering data  s, nm-1
ln I(s)
Cell division protein FtsQ Guinier plot ln 2.23×10-2 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Cell division protein FtsQ Kratky plot 1.104 0 3 sRg
p(r)
Cell division protein FtsQ pair distance distribution function Rg: 2.6 nm 0 Dmax: 8.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of the N-terminal soluble domain of cell division protein FtsQ in 20 mM phosphate buffer, 25 mM NaCl, pH 6.5 were collected on the 5-ID-D DND-CAT beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Rayonix LX170HS detector at a sample-detector distance of 0.2 m and at a wavelength of λ = 0.12398 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 1.5 mg/ml were measured at 21°C. Eight successive 1.150 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Cell division protein FtsQ (FtsQ)
Mol. type   Protein
Organism   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state   Monomer
Mon. MW   11.4 kDa
 
UniProt   P9WNA1 (1-99)
Sequence   FASTA