A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria.

Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE, Nat Commun 15(1):3167 (2024) Europe PMC

SASDRD5 – A ssr1698 Dri1 hemoprotein, H79A:R90A variant + heme

Ssr1698 protein (H79A:R90A)

MW^experimental	20	kDa
MW^expected	22	kDa
V^Porod	28	nm³

log I(s) 3.87×10⁰ 3.87×10^-1 3.87×10^-2 3.87×10^-3

Ssr1698 protein (H79A:R90A) small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Ssr1698 protein (H79A:R90A) Guinier plot

ln 3.88×10⁰ R_g: 2.0 nm 0 (2.0 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 2.0 nm 0 D_max: 6.3 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.3

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.102
0.914

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Ssr1698 protein (H79A:R90A) OTHER [STATIC IMAGE] model

Synchrotron SAXS data from solutions of a ssr1698 Dri1 hemoprotein, H79A:R90A variant + heme in 50 mM Hepes, 200 mM NaCl, pH 7.5 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II, Upton, NY, USA) using Pilatus3 X 1M and Pilatus3 X 900K duel detectors at sample-detector distances of 3.732 and 0.363 m, respectively, and at a wavelength of λ = 0.08199 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 80.00 μl sample was injected at a 0.45 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 4°C. 11 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ssr1698 protein (H79A:R90A)
Mol. type		Protein
Organism		Synechocystis sp. (strain PCC 6803 / Kazusa)
Olig. state		Dimer
Mon. MW		11.1 kDa

UniProt		P73129 (1-96)
Sequence		FASTA