Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP.

Kornilov FD, Slonimskiy YB, Lunegova DA, Egorkin NA, Savitskaya AG, Kleymenov SY, Maksimov EG, Goncharuk SA, Mineev KS, Sluchanko NN, Commun Biol 6(1):471 (2023) Europe PMC

SASDRG8 – Astaxanthin-binding protein orange1 (AstaPo1) complexed with zeaxanthin

Astaxanthin binding fasciclin family protein
MWexperimental 21 kDa
MWexpected 22 kDa
VPorod 43 nm3
log I(s) 8.70×101 8.70×100 8.70×10-1 8.70×10-2
Astaxanthin binding fasciclin family protein small angle scattering data  s, nm-1
ln I(s)
Astaxanthin binding fasciclin family protein Guinier plot ln 8.70×101 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Astaxanthin binding fasciclin family protein Kratky plot 1.104 0 √3 sRg
Dmax: 8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Astaxanthin binding fasciclin family protein PYMOL model
Synchrotron SAXS data from solutions of AstaPo1 complexed with zeaxanthin in 20 mM Tris-HCl, 150 mM NaCl, pH 7.6 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.05 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Zeaxanthin: https://pubchem.ncbi.nlm.nih.gov/compound/Zeaxanthin

Astaxanthin binding fasciclin family protein (AstaPo1)
Mol. type   Protein
Organism   Coelastrella astaxanthina
Olig. state   Monomer
Mon. MW   21.6 kDa
 
UniProt   S6BQ14 (21-223)
Sequence   FASTA