Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection.

Spiga L, Fansler RT, Perera YR, Shealy NG, Munneke MJ, David HE, Torres TP, Lemoff A, Ran X, Richardson KL, Pudlo N, Martens EC, Folta-Stogniew E, Yang ZJ, Skaar EP, Byndloss MX, Chazin WJ, Zhu W, Cell Host Microbe 31(10):1639-1654.e10 (2023) Europe PMC

SASDRH2 – Ligand bound Xenosiderophore Utilization System B (XusB)

DUF4374 domain-containing protein
MWI(0) 44 kDa
MWexpected 46 kDa
VPorod 77 nm3
log I(s) 1.11×101 1.11×100 1.11×10-1 1.11×10-2
DUF4374 domain-containing protein small angle scattering data  s, nm-1
ln I(s)
DUF4374 domain-containing protein Guinier plot ln 1.12×101 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
DUF4374 domain-containing protein Kratky plot 1.104 0 √3 sRg
p(r)
DUF4374 domain-containing protein pair distance distribution function Rg: 2.4 nm 0 Dmax: 7.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
DUF4374 domain-containing protein PYMOL model
Synchrotron SAXS data from solutions of ligand bound XusB in 20 mM Tris 150 mM NaCl, pH 8 were collected on the 12-ID-B beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 300K detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 2 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex KW-803 column at 25°C. 650 successive 0.350 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

DUF4374 domain-containing protein (XusB)
Mol. type   Protein
Organism   Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
Olig. state   Monomer
Mon. MW   46.4 kDa
 
UniProt   Q8A622 (41-464)
Sequence   FASTA