Synchrotron SAXS
data from solutions of
Tissue Transglutaminase + Ca: Time-resolved 100 ms
in
20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH 7.5
were collected
on the
ID7A1 BioSAXS / HP-Bio Beamline beam line
at the Cornell High Energy Synchrotron Source (CHESS) storage ring
(Ithaca, NY, USA)
using a Eiger 4M detector
at a wavelength of λ = 0.10972 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 1.00 mg/ml was measured
at 20°C.
15 successive
5 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
2 mg/mL ttG + 4 mM CaCl2 were rapidly mixed together with a Kenics style chaotic advection mixer. Final concentration during observation is 1 mg/mL ttG + 2 mM CaCl2. Molecular weight from Vc.
s, nm-1
