The acidic intrinsically disordered region of the inflammatory mediator HMGB1 mediates fuzzy interactions with CXCL12.

Mantonico MV, De Leo F, Quilici G, Colley LS, De Marchis F, Crippa M, Mezzapelle R, Schulte T, Zucchelli C, Pastorello C, Carmeno C, Caprioglio F, Ricagno S, Giachin G, Ghitti M, Bianchi ME, Musco G, Nat Commun 15(1):1201 (2024) Europe PMC

SASDRH9 – High mobility group protein B1, HMGB1

High mobility group protein B1 (D189E, E202D, E215D)
MWexperimental 28 kDa
MWexpected 25 kDa
VPorod 53 nm3
log I(s) 4.39×101 4.39×100 4.39×10-1 4.39×10-2
High mobility group protein B1 (D189E, E202D, E215D) small angle scattering data  s, nm-1
ln I(s)
High mobility group protein B1 (D189E, E202D, E215D) Guinier plot ln 4.40×101 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
High mobility group protein B1 (D189E, E202D, E215D) Kratky plot 1.104 0 √3 sRg
p(r)
High mobility group protein B1 (D189E, E202D, E215D) pair distance distribution function Rg: 2.6 nm 0 Dmax: 8.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
High mobility group protein B1, HMGB1 Rg histogram Rg, nm
Synchrotron SAXS data from solutions of HMGB1 in 20 mM Tris, 50 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 20°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

High mobility group protein B1 (D189E, E202D, E215D) (HMGB1)
Mol. type   Protein
Organism   Rattus norvegicus
Olig. state   Monomer
Mon. MW   24.7 kDa
 
UniProt   P63159 (2-215)
Sequence   FASTA