Compact polyethylenimine-complexed mRNA molecules as quintessential vaccines

Martin Schroer.

SASDRL9 – single self-amplifying RNA + 50 mM NaCl

self-amplifying RNA
MWI(0) 3300 kDa
MWexpected 3030 kDa
VPorod 112000 nm3
log I(s) 1.28×107 1.28×106 1.28×105 1.28×104
self-amplifying RNA small angle scattering data  s, nm-1
ln I(s)
self-amplifying RNA Guinier plot ln 1.29×107 Rg: 30.7 nm 0 (30.7 nm)-2 s2
(sRg)2I(s)/I(0)
self-amplifying RNA Kratky plot 1.104 0 √3 sRg
p(r)
self-amplifying RNA pair distance distribution function Rg: 32.7 nm 0 Dmax: 107.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
self-amplifying RNA DAMMIF model
Synchrotron SAXS data from solutions of single self-amplifying RNA in 5% w/v D-Glucose, 10mM MES (2-(N-morpholino)ethanesulfonic acid), 50 mM NaCl in double distillated sterile/RNAse free water, pH 6.1 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123985 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.20 mg/ml was measured at 23°C. 20 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Self-amplifying RNA (saRNA) in presence of 50 mM NaCl resulting in a mild level of compaction. DAM volume: 26.9 * 10³ nm³. MW: 3.3 MDa (monomer).

self-amplifying RNA (saRNA)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   3030 kDa
Sequence   FASTA