Synchrotron SAXS data from solutions of lysozyme in 40 mM sodium acetate, 50 mM NaCl, pH 4 were collected on the 13A beam line at the Taiwan Photon Source (NSRRC, Hsinchu, Taiwan) using a Eiger X 1M and Eiger X 9M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.083 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 10 mg/ml was injected at a 0.35 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 10°C. Six successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SAXS data shows influence of repulsive interparticle interference.
s, nm-1
