Structural and functional analysis of a plant nucleolar RNA chaperone-like protein.

Fernandes R, Ostendorp A, Ostendorp S, Mehrmann J, Falke S, Graewert MA, Weingartner M, Kehr J, Hoth S, Sci Rep 13(1):9656 (2023) Europe PMC

SASDRN5 – Nucleolar RNA Chaperone-Like Protein 1 (NURC1)

AT5g04600/T32M21_200
MWexperimental 34 kDa
MWexpected 25 kDa
VPorod 66 nm3
log I(s) 2.06×102 2.06×101 2.06×100 2.06×10-1
AT5g04600/T32M21_200 small angle scattering data  s, nm-1
ln I(s)
AT5g04600/T32M21_200 Guinier plot ln 2.07×102 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
AT5g04600/T32M21_200 Kratky plot 1.104 0 √3 sRg
p(r)
AT5g04600/T32M21_200 pair distance distribution function Rg: 3.7 nm 0 Dmax: 12.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
AT5g04600/T32M21_200 GASBOR model
log I(s)
 s, nm-1
AT5g04600/T32M21_200 SREFLEX model
log I(s)
 s, nm-1
AT5g04600/T32M21_200 ALPHAFOLD PROTEIN STRUCTURE DATABASE model
Synchrotron SAXS data from solutions of Nucleolar RNA Chaperone-Like Protein 1 (NURC1) in 50 mM HNa2PO4, 300 mM NaCl, 5% glycerol (v/v), 1 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 87.00 μl sample at 18 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. 2887 successive 1 second frames were collected through the entire SEC elution, where 92 data frames were selected from the SEC sample peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

AT5g04600/T32M21_200 (NURC1)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Monomer
Mon. MW   25.3 kDa
 
UniProt   Q9LZ65 (1-222)
Sequence   FASTA
 
ALPHAFOLD ID   Q9LZ65
 
ALPHAFOLD ID   Q9LZ65