Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles

Ehrit J, Gräwert T, Göddeke H, Konarev P, Svergun D, Nagel N, Biophysical Journal (2023) DOI

SASDRN9 – Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml)

Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml)
MWexperimental 42 kDa
MWexpected 45 kDa
log I(s) 5.23×10-3 5.23×10-4 5.23×10-5 5.23×10-6
Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) small angle scattering data  s, nm-1
ln I(s)
Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) Guinier plot ln 5.23×10-3 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) Kratky plot 1.104 0 √3 sRg
p(r)
Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) pair distance distribution function Rg: 3.4 nm 0 Dmax: 8.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) OTHER [STATIC IMAGE] model
log I(s)
 s, nm-1
Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) OTHER model
Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) OTHER model
Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) OTHER model
Synchrotron SAXS data from solutions of Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) in aqueous solution of 4% methanol, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 40 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Polysorbate 20: https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:53424

Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) (PS20)
Mol. type   Other
Olig. state   0
Mon. MW   1.2 kDa
Chemical formula