Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles

Ehrit J, Gräwert T, Göddeke H, Konarev P, Svergun D, Nagel N, Biophysical Journal (2023) DOI

SASDRP9 – Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml)

Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml)
MWexperimental 42 kDa
MWexpected 45 kDa
log I(s) 4.75×10-3 4.75×10-4 4.75×10-5 4.75×10-6
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) small angle scattering data  s, nm-1
ln I(s)
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) Guinier plot ln 4.76×10-3 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) Kratky plot 1.104 0 √3 sRg
p(r)
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) pair distance distribution function Rg: 3.5 nm 0 Dmax: 8.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) OTHER [STATIC IMAGE] model
log I(s)
 s, nm-1
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) OTHER model
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) OTHER model
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) OTHER model
Synchrotron SAXS data from solutions of Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) in aqueous solution of 4% methanol, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 40 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tetradecanoic acid (myristic acid): https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:28875 Polysorbate 20: https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:53424

Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) (PS20_HMA)
Mol. type   Other
Olig. state   0
Mon. MW   1.2 kDa
Chemical formula