| MWexperimental | 880 | kDa |
| MWexpected | 878 | kDa |
| VPorod | 927 | nm3 |
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log I(s)
9.52×10-1
9.52×10-2
9.52×10-3
9.52×10-4
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s, nm-1
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I-Shaped Dimers of a Plant Chloroplast FOF1-ATP Synthase in Response to Changes in Ionic Strength
Osipov S, Ryzhykau Y, Zinovev E, Minaeva A, Ivashchenko S, Verteletskiy D, Sudarev V, Kuklina D, Nikolaev M, Semenov Y, Zagryadskaya Y, Okhrimenko I, Gette M, Dronova E, Shishkin A, Dencher N, Kuklin A, Ivanovich V, Uversky V, Vlasov A, International Journal of Molecular Sciences 24(13):10720 (2023) DOISASDRR8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea purified by anion-exchange chromatography (~ 300 mM NaCl)
ATP synthase subunit alpha, chloroplasticATP synthase subunit beta, chloroplastic
ATP synthase gamma chain, chloroplastic
ATP synthase delta chain, chloroplastic
ATP synthase epsilon chain, chloroplastic
ATP synthase subunit a, chloroplastic
ATP synthase subunit b, chloroplastic
ATP synthase subunit b', chloroplastic
ATP synthase subunit c, chloroplastic
4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside
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Fits and models
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SAXS data from solutions of chloroplast FOF1-ATP synthase purified by anion-exchange chromatography in ~300 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH 8 were collected using a Rigaku MicroMax 007-HF instrument at the Moscow Institute of Physics and Technology (MIPT; Dolgoprudny, Russian Federation) equipped with a Multiwire gas-filled ASM DTR Triton 200 detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. One 5400 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The protein of study is a chloroplast FOF1-ATP synthase (cFOF1) from Spinacia oleracea solubilized in 0.04% (w/v) 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside (tPCC-α-M). SAXS data confirm that cFOF1 purified by anion-exchange chromatography (elution at ~300 mM NaCl) is a momomer with detergent belt including 517 detergent molecules. Taking into account a detergent belt allows improving the χ2 of the fit from 1.35 to 1.15. |
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