| MWexperimental | 186 | kDa |
| MWexpected | 173 | kDa |
| VPorod | 307 | nm3 |
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log I(s)
5.77×10-2
5.77×10-3
5.77×10-4
5.77×10-5
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s, nm-1
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A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis.
Pierson E, De Pol F, Fillet M, Wouters J, Commun Biol 6(1):1024 (2023) Europe PMCSASDRT4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) tetramer
Mycobacterium tuberculosis phosphoserine phosphatase SerB2|
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Fits and models
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Synchrotron SAXS data from solutions of Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) tetramer in 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH 7.4 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 14.5 mg/ml was injected at a 0.50 ml/min flow rate onto a BioResolve SEC mAb 200Å 2.5 µm, 7.8 x 300 mm column at 10°C. 15 successive 1 second frames were collected through the sample elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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