Synchrotron SAXS data from solutions of Mycobacterium marinum phosphoserine phosphatase (MmSerB2) dimer in 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH 7.4 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 12.8 mg/ml was injected at a 0.50 ml/min flow rate onto a BioResolve SEC mAb 200Å 2.5 µm, 7.8 x 300 mm column at 10°C. 11 successive 1 second frames were collected through the sample elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
