| MWexperimental | 126 | kDa |
| MWexpected | 122 | kDa |
| VPorod | 428 | nm3 |
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log I(s)
6.89×10-2
6.89×10-3
6.89×10-4
6.89×10-5
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s, nm-1
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Regulation of the Escherichia coli paaR2-paaA2-ParD2 toxin-antitoxin system
Marusa Prolic Kalinsek.SASDRV6 – Transcription regulator PaaR2
Phage repressor protein CI (C120S)|
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Fits and models
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Synchrotron SAXS data from solutions of transcription regulator PaaR2 in 20 mM Tris-HCl, 200 mM NaCl, pH 8 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2.2 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 10 mg/ml was injected at a 0.30 ml/min flow rate onto a Shodex KW402.5-4F column at 15°C. 630 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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