Structural-function relationship of YdaS, a Cro-type repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7

Marusa Prolic Kalinsek.

SASDRW6 – YdaS, a cro-type repressor from Escherichai coli O157:H7 cryptic prophage CP-933

Phage antirepressor protein Cro
MWexperimental 14 kDa
MWexpected 12 kDa
VPorod 19 nm3
log I(s) 1.63×10-2 1.63×10-3 1.63×10-4 1.63×10-5
Phage antirepressor protein Cro small angle scattering data  s, nm-1
ln I(s)
Phage antirepressor protein Cro Guinier plot ln 1.64×10-2 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Phage antirepressor protein Cro Kratky plot 1.104 0 √3 sRg
p(r)
Phage antirepressor protein Cro pair distance distribution function Rg: 2.2 nm 0 Dmax: 7.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Phage antirepressor protein Cro PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of YdaS from Escherichai coli O157:H7 cryptic prophage CP-933 in 20 mM sodium acetate, 150 mM NaCl, 1 mM TCEP, pH 5.5 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 10 mg/ml was injected at a 0.30 ml/min flow rate onto a Shodex KW402.5-4F column at 15°C. 600 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Phage antirepressor protein Cro (YdaS)
Mol. type   Protein
Organism   Escherichia coli O157:H7
Olig. state   Monomer
Mon. MW   11.9 kDa
 
UniProt   Q8XAD7 (1-100)
Sequence   FASTA
 
PDB ID   8C7K